Standard Operating Procedures
Every SOP that applies in this lab. UCLA revises its standard SOPs, so those are linked here, never copied — always open the official EH&S version rather than a saved one.
→ All UCLA EH&S laboratory documents · All UCLA EH&S biosafety documents · Safety Data Sheets
Lab-written chemical SOPs
Four hazards in this lab have no UCLA band. These SOPs are written by the lab and cover the gap. Each one names the UCLA banded SOP it supplements — read both.
| SOP | Covers | Read with |
|---|---|---|
| Chemical Sensitizers | H334 respiratory and H317 skin sensitizers — glutaraldehyde, ammonium persulfate, G 418, DNase, transfer buffer, FITC-dextran | Oxidizers · Corrosives · Carcinogens |
| Sodium Azide | Solid azide and every azide-preserved antibody stock. Metal azides, drain ban | Acute Toxicants |
| Guanidinium Salt Lysis Reagents | QIAzol, TRIzol, RLT, AL/AW1. Bleach contact releases cyanide gas | Phenol · Acutely Toxic Corrosives |
| Potent Bioactive Research Compounds | Bafilomycin, Y-27632, DAPT, CCCP. DMSO stocks as systemic exposure | Novel Chemicals with Unknown Hazards |
These are laboratory-written, not EH&S templates, and are pending EH&S review before filing in the Chemical Hygiene Plan.
UCLA banded chemical SOPs
Assigned from this lab’s chemical inventory. Sixteen are required by chemicals actually held; three are included because the hazard exists but is not tracked in the chemical inventory.
Required
| SOP | Driven by |
|---|---|
| Flammables | 17 chemicals |
| Carcinogens and Reproductive Toxicants | 10 chemicals |
| Corrosives | 9 chemicals |
| Acute Toxicants | 6 chemicals |
| Formaldehyde | 5 chemicals |
| Corrosive Flammables | 2 chemicals |
| Oxidizing Corrosives | 2 chemicals |
| Acutely Toxic Corrosives | QIAzol · formaldehyde 37% |
| Peroxide Forming Chemicals | 2 chemicals |
| Novel Chemicals with Unknown Hazards | 2 chemicals |
| Oxidizers | 1 chemical |
| Phenol | QIAzol |
| Dichloromethane | 1 chemical. Its direct Box link requires a login — this goes to the EH&S index instead |
| Halogenated Solvents | 1 chemical |
| Acutely Toxic Chlorinated Solvents | Chloroform |
| Acutely Toxic Carcinogens and Reproductive Toxicants | Azoxymethane |
Chemical-specific SOPs override the generic band. Where Formaldehyde, Phenol or Dichloromethane applies, it is the one to follow.
Also in force
Hazards present in the lab that the chemical inventory does not track.
| SOP | Why |
|---|---|
| Liquid Nitrogen and other Cryogens | LN2 in MRL 1529 |
| Compressed Gases | CO₂ cylinders |
| Anesthetics | Isoflurane |
Biosafety — what applies here
The lab works with adeno-associated viral vectors, third-generation HIV-based lentiviral vectors, unfixed human intestinal, brain and blood-derived specimens, human and mouse cell lines and organoid cultures, and transgenic mice.
General practice — incident response · biohazardous spill response · donning and doffing PPE · hand sanitation · transport of biohazardous materials. Decontamination is the one exception: the lab follows its own modified copy of the EH&S SOP, which sets the minimum contact time for 10% bleach at 10 minutes. Ask the PI for it rather than using the EH&S version.
Equipment and technique — biosafety cabinet use · sharps use · centrifugation · aerosol-generating equipment · aspiration flask · ELISA · imaging live cells · handling human materials outside a biosafety cabinet
Animal work — AAV in rodents · biohazardous animal injections · handling and restraint · necropsy · cage changing and washing · transport · ABSL-2 containment · laboratory animal allergies
Waste — solid medical waste · liquid medical waste · sharps medical waste · recombinant nucleic acid waste · pathology waste · biohazardous animal disposal
All of these are at ehs.ucla.edu/documents/biosafety.
Lab-written biosafety SOPs
The lab’s own vector, human-specimen, incident-response and transport SOPs carry room-level detail and personal contact numbers, so they are not published here. Lab members: they are linked from the Slack canvas in #lab-safety, and the signed copies are in the safety manual.
They cover lentiviral vectors, AAV in rodents, human tissue and blood-derived samples, biohazardous incident response, and transport of biohazardous materials — plus the lab’s modified Decontamination SOP, the completed Bloodborne Pathogen Exposure Control Plan, and the lentivirus Post-Exposure Plan, which is the only Post-Exposure Plan this lab holds. Lab members: the current versions are the ones in the lab’s Google Drive SOP folder.
Reference
| UCLA Chemical Hygiene Plan | Listed on the EH&S laboratory documents page. Cal/OSHA requires it be readily available wherever hazardous chemicals are used, handled or stored |
| UCLA Institutional Biosafety Plan | The institutional plan the lab-specific biosafety manual sits under |
| Safety Data Sheets | UC Risk & Safety Solutions |
| Research Safety Training Guide | The course matrix behind the training page |
Chemical SOP assignments from the lab’s inventory review, 19 August 2026. Re-run when the inventory changes materially. Biosafety section reviewed 27 August 2026 against the lab’s Biosafety Use Authorization.